CRITERION™ Mannitol Salt Agar (MSA), Dehydrated Culture Media, 500g Wide-Mouth Bottle

SKU: C6231
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CRITERION™ Mannitol Salt Agar (MSA) is a selective and differential culture medium designed for the isolation and differentiation of pathogenic Staphylococcus species, particularly Staphylococcus aureus, from clinical, food, and environmental samples.

MSA uses a high sodium chloride concentration (7.5%) to inhibit the growth of most other bacteria while allowing halotolerant Staphylococci to thrive. The presence of mannitol and phenol red enables differentiation: pathogenic S. aureus ferments mannitol, producing acid that changes the medium’s color from red to yellow, while non-pathogenic Staphylococcus epidermidis does not.

The CRITERION™ line by Hardy Diagnostics offers exceptional quality, consistency, and ease of use. The wide-mouth, easy-grip 500g bottle simplifies handling and reduces contamination risk, while the high-purity formulation ensures reproducible growth results across testing applications.

Features and Benefits:
• Selective for halotolerant bacteria, especially Staphylococcus spp.
• Differential color change to distinguish S. aureus (yellow colonies) from non-pathogenic species (red colonies)
• Ideal for use in clinical, food, and cosmetic microbiology
• Supplied as dehydrated powder for customizable batch preparation
• Wide-mouth bottle reduces dust and spillage during dispensing
• Fast-dissolving formula for convenient media preparation
• Available in multiple container sizes (500g, 2kg, 10kg, and 2L pre-measured Mylar® bags)
• Manufactured under ISO 13485 and cGMP quality systems for reliable performance

CRITERION™ Mannitol Salt Agar (MSA) is a dehydrated culture medium used for the selective isolation and differentiation of Staphylococcus spp., particularly S. aureus. High salt concentration and mannitol fermentation enable rapid identification. Supplied in a 500g wide-mouth bottle for ease of use. Manufactured by Hardy Diagnostics under cGMP and ISO 13485 standards.

Specifications

Product Type: Dehydrated Culture Media
Medium Name: Mannitol Salt Agar (MSA)
Intended Use: Isolation and differentiation of Staphylococcus spp.
Principle:

Sodium chloride (7.5%) provides selectivity by inhibiting non-halotolerant organisms.

Mannitol serves as a fermentable carbohydrate source.

Phenol red acts as a pH indicator to detect mannitol fermentation.
Typical Composition (per liter):

Beef Extract: 1.0 g

Peptone: 10.0 g

Sodium Chloride: 75.0 g

Mannitol: 10.0 g

Phenol Red: 0.025 g

Agar: 15.0 g
pH (at 25°C): 7.4 ± 0.2
Appearance: Light beige, free-flowing powder
Fill Volume: 500g
Container Type: Wide-mouth, easy-grip plastic bottle
Storage Temperature: 10–30°C (dry environment)
Shelf Life: 3 years (unopened)
Applications:

Isolation and identification of Staphylococcus aureus from food, water, and surfaces

Quality control testing in cosmetics and personal care manufacturing

Microbiological safety testing in cannabis and food labs
Industries:
Breweries, Wineries & Distilleries, Cannabis QA Testing, Cosmetics, Environmental, Food Safety

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