4-Aminoantipyrine, Reagent Grade | Glucose Determination Reagent | Go Green Scientific

4-Aminoantipyrine - 10G

SKU: A4382-10G
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4-Aminoantipyrine
reagent grade

Synonym(s): 4-Amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one, Ampyrone

Description

4-Aminoantipyrine (also known as Ampyrone or 4-Amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one) is a high-purity reagent widely used in enzymatic glucose determination methods. When combined with phenol and peroxidase, it forms a chromogenic complex suitable for spectrophotometric quantification of glucose in food, beverage, and general analytical workflows.

This reagent grade material provides reliable performance, excellent lot consistency, and high solubility, making it suitable for routine analysis, quality control, and research applications where accuracy and reproducibility are essential.

Application
Reagent for glucose determination in the presence of phenol and peroxidase.

Key Features
• Reagent-grade chromogenic compound used in enzymatic glucose assays
• Forms a quinoneimine dye in the presence of phenol and peroxidase (GOD–POD system)
• Suitable for photometric quantification of glucose in food, beverage, and general analytical applications
• High-purity (≥97% HPLC) powder for reliable assay performance
• Soluble in water at 50 mg/mL for convenient reagent preparation

Overview
4-Aminoantipyrine (Ampyrone) is a high-purity reagent widely used in glucose determination assays. In the glucose oxidase–peroxidase (GOD–POD) reaction system, 4-aminoantipyrine reacts with phenol and hydrogen peroxide to form a chromogenic quinoneimine dye, enabling sensitive photometric measurement. This reagent is commonly employed in food testing, beverage analysis, and clinical-style enzymatic assays requiring accurate colorimetric glucose quantification.

Specifications
Empirical Formula (Hill Notation): C11H13N3O
CAS Number: 83-07-8
Molecular Weight: 203.24
Beilstein/REAXYS Number: 181635
EC Number: 201-452-3
MDL Number: MFCD00003145
UNSPSC Code: 51384603
PubChem Substance ID: 24890855
NACRES: NA.31
Grade: Reagent grade
Quality Level: 300
Assay: ≥97% (HPLC)
Form: Powder
Melting Point: 105–110 °C (lit.)
Solubility: Water, 50 mg/mL
SMILES: CN1N(c2ccccc2)C(=O)C(N)=C1C
InChI: 1S/C11H13N3O/c1-8-10(12)11(15)14(13(8)2)9-6-4-3-5-7-9/h3-7H,12H2,1-2H3
InChI Key: RLFWWDJHLFCNIJ-UHFFFAOYSA-N

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