Glucose oxidase/peroxidase reagent
for use with enzymatic assay kits GAGO20, STA20

Synonym(s): GOD-POD Reagent

Description

Glucose Oxidase/Peroxidase Reagent (GOD–POD) is a ready-to-use enzymatic reagent blend designed for the quantitative determination of glucose in biological, food, beverage, fermentation, and research samples. This reagent is used in combination with O-dianisidine (or comparable chromogenic substrates) within enzymatic colorimetric glucose assays.
The reagent contains glucose oxidase (GOD) and horseradish peroxidase (POD), enabling a two-step coupled reaction. Glucose oxidase converts β-D-glucose to D-glucono-δ-lactone and hydrogen peroxide; peroxidase then catalyzes the oxidation of a chromogenic substrate, generating a measurable colored product suitable for photometric detection.
This reagent is fully compatible with Sigma’s GAGO20 and STA20 assay kits and is widely used for determining glucose levels in plasma, serum, urine, and general analytical applications.

Application
Glucose oxidase/peroxidase reagent along with O-Dianisidine was used for the determination of blood and urinary glucose, especially the presence of fructose for both research and clinical purpose.[1] It may be used for determining glucose in plasma or serum.[2] It may also be used in the estimation of blood glucose as a function of the oxidative coupling system or of the oxygen affinity of the oxygen acceptor chromogen.