Methanol ≥99.9% HPLC Grade Solvent, 100 mL–4x4 L

Methanol, Gradient Grade, ≥99.9% (HPLC & Immunofluorescence Suitable) - 2L

SKU: 34885-2L-R
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High-purity methanol (CH₃OH) gradient grade, ≥99.9%, suitable for HPLC and immunofluorescence applications. Filtered through a 0.2 µm filter to ensure low particulate content and optimized UV transparency for analytical workflows.

This solvent meets stringent impurity and absorbance specifications required for gradient HPLC analysis, reversed-phase separations, and fluorescence detection methods. Low water content and controlled aldehyde/ketone levels support sensitive analytical assays, including biomolecule quantification and oxidative stress studies.

Commonly used as:

HPLC mobile phase component

LC-UV analytical solvent

Immunofluorescence assay reagent

Extraction solvent for food, environmental, and biological matrices

Designed for laboratories requiring reproducible chromatographic performance and controlled trace impurity levels.

Chemical Name: Methanol
Synonyms: Methyl alcohol; MeOH; Wood alcohol; Carbinol
CAS Number: 67-56-1
Molecular Formula: CH₃OH
Molecular Weight: 32.04 g/mol
Grade: Gradient grade
Assay: ≥99.9%
Form: Liquid
Filtration: 0.2 µm filtered
Application: HPLC, LC-UV, immunofluorescence, analytical extractions

SPECIFICATIONS

Methanol, Gradient Grade, ≥99.9%

Chemical Formula: CH₃OH

CAS Number: 67-56-1

Molecular Weight: 32.04 g/mol

Assay: ≥99.9%

Grade: Gradient grade

Form: Liquid

Quality Level: 100

Physical Properties

Vapor Density: 1.11 (vs air)

Vapor Pressure: 410 mmHg at 50 °C; 97.68 mmHg at 20 °C

Autoignition Temperature: 725 °F

Melting Point: −98 °C

Color: APHA ≤10

Evaporation Residue: <0.0003%

Impurity Limits

Free Alkali (as NH₃): ≤0.0005%

Non-volatile Matter: ≤0.0005%

Acetaldehyde: ≤0.001%

Acetone (GC): ≤0.001%

Formaldehyde: ≤0.001%

Water (Karl Fischer): ≤0.02%

Fluorescence (quinine) at 254 nm: ≤1 ppb

Fluorescence (quinine) at 365 nm: ≤1 ppb

HPLC Gradient Performance

≤2 mAU at 230 nm

≤5 mAU at 254 nm

UV Absorbance Maximum

210 nm: 0.50

220 nm: 0.30

230 nm: 0.15

235 nm: 0.10

240 nm: 0.05

260 nm: 0.01

400 nm: 0.01

Preparation

Filtered through 0.2 µm membrane

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