o-Dianisidine Dihydrochloride | 5 mg Vial | Chromogenic GOD–POD Reagent

o-Dianisidine dihydrochloride 5MG

SKU: D2679-1VL
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o-Dianisidine dihydrochloride
vial of 5 mg

Synonym(s): o-Dianisidine reagent

Description

o-Dianisidine Dihydrochloride is a chromogenic substrate used in colorimetric glucose assays based on the glucose oxidase–peroxidase (GOD–POD) reaction. In the presence of glucose oxidase and horseradish peroxidase, o-dianisidine is oxidized by hydrogen peroxide to produce a measurable colored product, enabling sensitive detection of glucose in food, beverages, fermentation samples, biological fluids, and general analytical workflows.

This material is provided as a 5 mg solid reagent suitable for photometric detection at 430–540 nm (depending on the oxidized chromophore). It is widely used in assay kits and laboratory protocols requiring rapid, reliable, and high-contrast color development.

Application
Indicator substrate for the quantitative determination of glucose in food and other materials utilizing the glucose oxidase-peroxidase-o-dianisidine enzymatic assay.

Key Features
• Chromogenic substrate for glucose oxidase–peroxidase (GOD–POD) assays
• Provides strong colorimetric signal for quantitative glucose detection
• Supplied as a 5 mg solid reagent in a sealed vial
• Suitable for photometric measurement in food, beverage, and biological samples
• Compatible with enzymatic assays for glucose and carbohydrate analysis
• Ships and stores at 2–8°C

Applications
• Quantitative glucose determination in food and beverage samples
• General analytical carbohydrate detection
• Clinical or research glucose assays using GOD–POD chemistry
• Photometric methods requiring oxidative coupling color development

Storage & Handling
• Store at 2–8°C
• Protect from light and moisture
• Prepare working solutions fresh as needed

Notes
• Used as the chromogenic component in the glucose oxidase–peroxidase–o-dianisidine reaction
• Forms colored oxidized species measurable at 430–540 nm depending on assay conditions

Specifications
EC Number: 243-737-5
Form: Solid
Packaging: Vial of 5 mg
Technique: Photometry (suitable)
Applications: Food and beverages; general analytical assays
Storage Temperature: 2–8°C
UNSPSC Code: 12164500
NACRES: NA.84

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