Supelco® 37 Component FAME Mix is a certified reference material (CRM) containing a mixture of fatty acid methyl esters (FAMEs) prepared in dichloromethane. This TraceCERT® multicomponent calibration standard is designed for chromatographic analysis of fatty acid methyl esters using gas chromatography (GC) or high-performance liquid chromatography (HPLC).

FAME reference standards are widely used in analytical laboratories for calibration, identification, and quantification of fatty acids in food products, oils, and biological samples. Multicomponent FAME mixtures provide reliable retention time markers and calibration references when performing fatty acid profiling using GC, GC-FID, or GC-MS methods.

The Supelco 37 Component FAME Mix is widely used in food and beverage laboratories for analysis of fatty acid composition, including omega-3 fatty acids in fish and other food matrices. These standards support routine quality control, method development, and regulatory testing of fatty acid profiles in edible oils, nutritional products, and biological samples.

This CRM contains a certified mixture of 37 fatty acid methyl ester analytes including short-, medium-, and long-chain saturated and unsaturated fatty acid methyl esters commonly found in food and biological lipid samples.

Representative analytes include:

Methyl butyrate

Methyl hexanoate

Methyl octanoate

Methyl decanoate

Methyl undecanoate

Methyl laurate

Methyl tridecanoate

Methyl myristate

Methyl myristoleate

Methyl pentadecanoate

Methyl cis-10-pentadecenoate

Methyl palmitate

Methyl palmitoleate

Methyl heptadecanoate

cis-10-Heptadecenoic acid methyl ester

Methyl stearate

trans-9-Elaidic acid methyl ester

cis-9-Oleic acid methyl ester

Methyl linolelaidate

Methyl linoleate

Methyl arachidate

Methyl γ-linolenate

Methyl cis-11-eicosenoate

Methyl linolenate

Methyl heneicosanoate

cis-11,14-Eicosadienoic acid methyl ester

Methyl behenate

cis-8,11,14-Eicosatrienoic acid methyl ester

Methyl erucate

cis-11,14,17-Eicosatrienoic acid methyl ester

cis-5,8,11,14-Eicosatetraenoic acid methyl ester

Methyl tricosanoate

cis-13,16-Docosadienoic acid methyl ester

Methyl lignocerate

cis-5,8,11,14,17-Eicosapentaenoic acid methyl ester (EPA)

Methyl nervonate

cis-4,7,10,13,16,19-Docosahexaenoic acid methyl ester (DHA)

Component concentrations range from 200–600 µg/mL depending on analyte, as specified on the certificate of analysis.

TraceCERT® certified reference materials are produced and certified in accordance with ISO 17034 and ISO/IEC 17025 quality systems, ensuring traceability and analytical reliability. Each standard is supplied with a certificate of analysis containing certified component values suitable for calibration and analytical quality control.

This certified reference material is supplied with a certificate of analysis providing traceable concentration values suitable for calibration, method validation, and analytical quality control.

Shipping and Storage Notice

Please note that shipping and long-term storage conditions for certified reference materials may differ. Manufacturers determine the most appropriate shipping method based on product stability and shipping data. Some CRM standards may ship at ambient temperature even though recommended long-term storage conditions are refrigerated or frozen. This does not affect product integrity when handled according to manufacturer documentation.