TWEEN® 80 skeletal structure

TWEEN® 80, for cell culture, 500 mL

SKU: P4780-500ML
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TWEEN® 80, 500 mL

suitable for cell culture, suitable for insect cell culture, viscous liquid

Synonym(s):
POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80

CAS Number: 9005-64-6
MDL Number: MFCD00082107
UNSPSC Code: 12161700
NACRES: NA 25

General description
TWEEN 80 is a polyethylene sorbitol ester[1] and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.[2]

Application
Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
TWEEN® 80 has been used:
• in Listeria monocytogenes enumeration using filtration method[3]
• as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion[4]
• as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing[5]

Legal Information
TWEEN is a registered trademark of Croda International PLC

Widely used non-ionic surfactant. Supports protein extraction, microbial culture (Listeria, BCG), and emulsion formation. Cell culture compatible. CAS 9005-64-6.

Properties:

Description: Non-ionic
Quality Level: 200
Form: Viscous Liquid
Mol Wt:
- average mol wt 1310
Composition: Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)
Techniques:
-Cell culture | insect: suitable
-Cell culture | mammalian: suitable
CMC: 0.012 mM
Density:
-1.06 g/mL at 25 °C (lit.)
-1.07 g/cm3 at 25 °C (lit.)
Application(s): (ELISA and immunoassays)
SMILES string: CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

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